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Objective:To compare the clinical application effect of polyetheretherketone (PEEK) and titanium in cranioplasty.Methods:Sixty patients with skull loss in Shengjing Hospital of China Medical University from January 2015 to December 2019 were retrospectively analyzed. Among them, 25 patients were repaired with PEEK material (PEEK group) and 35 patients were repaired with titanium mesh (titanium mesh group). The postoperative complications within 6 months, plastic surgery satisfaction, hospital stay and hospitalization cost were compared between 2 groups.Results:There were no significant differences in bleeding, infection, epilepsy, implant displacement, implant exposure, incidence of secondary operations and the length of hospital stay between 2 groups ( P>0.05). The incidence of subcutaneous fluid, good plastic surgery satisfaction and hospitalization costs in the PEEK group were significantly higher than those in titanium mesh group: 28.0% (7/25) vs. 5.7% (2/35), 88.0% (22/25) vs. 62.9% (22/35) and (12.4 ± 1.5) × 10 4 yuan vs. (7.0 ± 2.4) × 10 4 yuan, and there were statistical differences ( P<0.05 or<0.01). Conclusions:PEEK material is safe and effective in cranioplasty, and the treatment satisfaction is better, but the cost is higher. PEEK can be used as an ideal skull repair material for patients with economic basis.
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Based on clinical training and practice experience of the authors in recent years, the training practice of critical ultrasonography in standardized training of neurosurgical specialists were summarized in the study. The study also discussed the appropriate training mode and training requirements of critical ultrasonography in standardized training of neurosurgical specialists, from the aspects of basic theory training, clinical application training of critical ultrasonography and special training of craniocerebral ultrasound, training precautions and training assessment, so as to provide comprehensive evaluation and treatment strategies for neurological intensive patients.
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Objective:To investigate the effect of constant temperature blankets on intravascular hypothermia for severe traumatic brain injury (sTBI). Methods:A total of 112 inpatients with sTBI from January, 2013 to December, 2018 were reviewed. They were divided into control group (n = 58) and observation group (n = 54) according to whether a self-warming blanket was used. They were assessed with Bedside Shivering Assessment Scale (BSAS). Their dosages of anti-shivering medicine, coagulation and intracranial pressure were recorded. The scores of Glasgow Outcome Scale Extended (GOSE) and the mortality one, six and twelve months after discharge were observed. Results:The incidence and severity were less in the observation group than in the control group (χ2 = 16.212, P < 0.01). The dosage of anti-shivering medicine was less in the observation group than in the control group (t > 1.269, P < 0.05). The hypercoagulation relieved significantly six hours after hypothermia in the observation group, and it was stable twelve hours after hypothermia. For the control group, the hypercoagulation relieved significantly twelve hours after hypothermia, and it was stable 24 hours after hypothermia. The intracranial pressure decreased more in the observation group than in the control group. The GOSE score and the mortality were less in the observation group than in the control group (t > 1.168, P < 0.05) one, six and twelve months after discharge. Conclusion:Application of self-warming blankets in intravascular hypothermia for sTBI may relieve shivering, hypercoagulation and intracranial pressure, to improve the outcome of patients.
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Objective: To study the clinical efficacy of modified Zhenwutang combined with Zhengji technique on cold-dampness arthralgia syndrome caused by knee osteoarthritis (KOA) at episode and the effect on inflammatory factors of joint fluid. Method: One hundred and forty-eight patients were randomly divided into control group and observation group by random number table. Patients in control group got celecoxib capsules, 0.2 g/time, 1 time/day, and Zhengji technique with lumbar positioning oblique pulling and finger pressing for 12 times, 1 time for every two days, 3 times/week. Patients in observation group got modified Zhenwutang, 1 dose/day, and the same Zhengji technique. The course of treatment was 4 weeks. Before and after treatment, western Ontario and McMaster University Osteoarthritis index (WOMAC), pain and swelling, index of severity of osteoarthritis (ISOA), local signs of knee joint and cold-dampness obstruction syndrome were scored, and the score of quality of life were discussed by arthritis impact measurement scale 2 (AIMS2-SF). And levels of interleukin-1 beta (IL-1β), IL-17, tumor necrosis factor-alpha (TNF-α), substance P (SP) and calcitonin gene-related peptide (CGRP) were detected. Result: The clinical efficacy in observation group was better than that in control group (Z=2.131, PPPβ, IL-17, TNF-α, SP and CGRP were higher than those in control group (PConclusion: Modified Zhenwutang combined with Zhengji technique can relieve clinical symptoms of patients with cold-dampness arthralgia syndrome caused by knee osteoarthritis (KOA) at episode, ameliorate joint function to improve patients' quality of life, reduce the expression of proinflammatory factors and neuropeptides in synovial fluid, so as to inhibit the inflammatory response and controlling clinical symptoms.
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<p><b>INTRODUCTION</b>Acute fatty liver of pregnancy (AFLP) frequently causes liver failure in pregnant women. A better understanding of the clinical characteristics, management, outcomes and risk factors of AFLP is required, given its relatively high mortality rate. We aimed to describe the characteristics of AFLP, and further assess its outcomes and potential risk factors from the perspectives of the mother and fetus.</p><p><b>METHODS</b>This was a retrospective cohort study of 133 patients with AFLP hospitalised at four tertiary hospitals in China between January 2009 and April 2014.</p><p><b>RESULTS</b>Among 133 patients, AFLP was diagnosed in the postpartum period for 13 (9.8%) patients. Potential factors influencing adverse maternal outcome were male fetus (p = 0.04), postpartum diagnosis of AFLP (p < 0.01), intrauterine fetal death (p = 0.04), disseminated intravascular coagulation (p < 0.01), prothrombin time (p < 0.01) and activated partial thromboplastin time (p = 0.04). The frequency of fetal distress (p = 0.03) and activated partial thromboplastin time (p < 0.05) were significantly higher in pregnancies with dead fetuses than in those where the fetuses survived. Independent risk factors for perinatal maternal mortality were history of legal termination of pregnancy (odds ratio [OR] 1.958, 95% confidence interval [CI] 1.133-3.385), total bilirubin (OR 1.009, 95% CI 1.003-1.014) and serum creatinine (OR 1.010, 95% CI 1.003-1.017).</p><p><b>CONCLUSION</b>Compared with total bilirubin and serum creatinine, history of legal termination of pregnancy appeared to be a greater risk factor for maternal mortality among patients with AFLP.</p>
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Objective To investigate the clinical diagnosis value of autoantibodies and inflammatory factors in lupus erythematosus.Methods 50 patients with lupus erythematosus in the hospital from January 2015 to October 2016 were selected as the observation group,which was further divided into the active and non active subgroups according to the disease activity index of lupus erythematosus.Meanwhile 45 women undergoing healthy physical examination were selected as the control group.The anti nuclear antibody(ANA),anti dsD-NA antibody and anti Sm antibody,anti nucleosome antibody(AnuA),anti rRNP antibody and interleukin 1 beta(IL)-1,IL-6,IL-8,and tumor necrosis factor alpha(TNF-α)levels were detected.The detection results were statistically analyzed.Results Compared with the control group,the positive rates of ANA,anti-dsD-NA antibody,anti Sm antibody,AnuA and anti rRNP antibody,and levels of serum IL-1β,IL-6,IL-8 and TNF-α in the observation group were significantly increased,the difference was statistically significant(P<0.05). Adopting 5-autoantibodies combined detection in lupus erythematosus,the positive rate in the observation group was 100.00%;compared with the inactive lupus erythematosus subgroup,the positive rates of anti dsD-NA antibody and AnuA in the active stage subgroup were significantly increased,moreover serum IL-1β,IL-6, IL-8 and TNF-α levels were significantly increased,the difference was statistically significant(P<0.05).Con-clusion Autoantibodies and inflammatory factors have a certain value in the early diagnosis of lupus erythe-matosus.T he sensitivity of autoantibodies combined detection is high.
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The training of neurocritical care is an important component in standardized training of neurosurgical specialists. As a subspeciality of neurosurgery, as well as with the characteristics of critical care medicine, neurocritical care carries its own discipline features. Based on clinical training and practice experiences, the training mode and practice experience were summarized, including basic requirement, theory training, skill training, research training, humanity accomplishment improvement and evaluation stan-dard, in order to discuss the appropriate training mode of neurocritical care in standardized training of neurosurgical specialists, improve the training methods and advance the training effectiveness, and provide reference for cultivating qualified and comprehensively developed neurosurgical specialists.
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Objective To investigate and compare the effect of two kinds of transparent dressings in the use of fixed intravenous indwelling needle in restless patients in emergency first aid. Methods A total of 160 restless patients who were required to use intravenous indwelling needle were randomly divided into conventional dressing group and 3M dressing group by random digits table method, 80 patients in each group. The medical transparent dressings(Specification:6 cm × 7 cm) were used for the patients of conventional dressing group, and 3M dressings(Specification:6.5 cm×7.0 cm ) for 3M dressing group. The fixity time, number of cases intravenous indwelling needle detached, replaced time of transparent dressing, number of crimping cases were compared. Results For the conventional dressing group, fixity time was (19.67 ± 7.02) h, replaced time was (18.92 ± 6.58) h, 15 detached cases and 24 crimping cases happened. While for the 3M dressing group, fixity time was (41.64±17.79) h, replaced time was (41.11 ± 17.76) h, 4 detached cases and 7 crimping cases happened. The difference was statistically significant(t=-10.27,χ2=7.23, t=-10.48,χ2=11.56, all P<0.05). Conclusions 3M transparent dressing is suggested in fixing intravenous indwelling needle in restless patient in emergency first aid. It can ensure the use of intravenous indwelling needle during the period, effectively prevents the needle from detaching due to its good fixation effect.
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Objective To evaluate the effect of air pollution on carbonylated protein level in the stratum corneum,and to assess the protective effect of pink pepper tree extracts and lipid mixtures on skin damage.Methods After the investigation of influencing factors in the preliminary experiment,fluorescence labelling assay was performed to detect the carbonylated protein level in the skin stratum corneum at different body sites of 34 healthy testees.Cigarette smoke was used to simulate pollutants,and the forearms of 15 healthy testees were exposed in the customized pollution simulation chamber with the flexor aspects facing upwards.After 0,1,2,4,5 hours of exposure,stratum corneum samples were collected by using D-squame tapes.In each of 14 selected healthy testees,3 adjacent areas on the flexor aspect of unilateral forearm were divided into 3 groups:pink pepper tree group treated with 1％ aqueous solution of pink pepper tree extracts,control group treated with deionized water,and blank group receiving no treatment.Then,the forearms of the 14 testees were exposed in the pollution simulation chamber for 5 hours,and stratum corneum samples were collected from the 3 areas before and after the exposure.Another 16 healthy testees were included,and 3 adjacent areas on the flexor aspect of their unilateral forearms were divided into 3 groups:lipid mixture group treated with 5％ lipid mixture emulsion,control group treated with lipid mixture-free placebo emulsion,and blank group receiving no treatment.Then,the forearms of the 16 testees were exposed in the pollution simulation chamber for 5 hours,and stratum corneum samples were collected from the 3 areas before and after the exposure.Moreover,twenty healthy testees were enrolled into the double-blind split-face clinical trial.That is,one half of the face was randomly chosen to be treated with 1％ emulsion of pink pepper tree extracts,and the other facial side was treated with placebo emulsion.Before and after 56-day treatment,stratum corneum samples were collected from the cheeks of testees by using D-squame tapes.Fluorescence labelling assay was conducted to detect the carbonylated protein level in the above stratum corneum samples.Results The analysis of 34 testees showed that carbonylated protein levels (average fluorescence intensity) significantly differed among different body sites (P ＜0.001),and the carbonylated protein levels were significantly higher in the cheeks (26.3 ± 7.1) and forehead (22.9 ± 7.9) than in the forearm (14.7 ± 4.9) and waist and back (12.6 ± 4.2) (P ＜ 0.001),and higher in the forearm than in the waist and back (P =0.046).In the short-term simulated accelerated exposure experiment,the carbonylated protein level increased along with the increase of the duration of exposure to pollution (R2 =0.995 9).After 5-hour exposure,the pink pepper tree group and lipid mixture group both showed significantly lower elevated levels of carbonylated protein in the stratum corneum (9.7 ± 5.2,5.8 ± 4.9) compared with the corresponding blank groups (19.0 ± 10.0,17.4 ± 8.8;P ＜ 0.005) and control groups (18.5 ± 7.3,15.9 ± 6.4;P ＜ 0.005).In the long-term human trial,the carbonylated protein levels significantly decreased in the facial side treated with 1％ emulsion of pink pepper tree extracts for 8 weeks compared with the placebo-treated facial side.Conclusion Air pollution aggravates skin damage induced by protein carbonylation in the stratum corneum,and pink pepper tree extracts and lipid mixtures can effectively reduce the carbonylated protein level.
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<p><b>OBJECTIVE</b>To evaluate the clinical effect of traditional Chinese spinal orthopedic manipulation (TCSOM) in treating patients with functional abdominal pain syndrome (FAPS) in comparison with Pinaverium Bromide (Dicetel, PBD), and to assess a possible cause for FAPS.</p><p><b>METHODS</b>Eighty patients with FAPS were randomly and equally assigned to the TCSOM group and PBD group according to the random number table. All patients in the TCSOM group were treated with a maximum of 5 times of spinal manipulations. Patients in the PBD group were instructed to take 50 mg 3 times a day, consistently for 2 weeks. The symptoms of pre- and post-treatment were assessed on a visual analog scale (VAS) pain score. A symptom improvement rating (SIR) was implemented to evaluate the effects of the treatments.</p><p><b>RESULTS</b>The symptoms of 27 cases of the TCSOM group were relieved soon after the first TCSOM treatment and 9 cases were significantly improved. The VAS pain scores in the TCSOM group were significantly lower than those in the PBD group after 2 weeks treatment. According to the SIR based on VAS, the TCSOM group included 30 cases with excellent results, 7 cases with good, and 3 cases with poor. Adverse events to the treatment were not reported. Based on VAS, the PBD group reported 8 cases with excellent results, 10 cases with good and 22 cases with poor. There was a significant difference between the two groups (P<0.01).</p><p><b>CONCLUSIONS</b>The displacement of intervertebral discs and/or vertebra in the thoracic or lumbar region seems to be a contributing factor in the symptoms of FAPS. TCSOM is an effective treatment for FAPS.</p>
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To summarize the clinical experience of Professor WANG Meng-yong in the treatment of uric acid nephropathy. Professor WANG believes that the disease is mainly caused by spleen and kidney deficiency, disorder of function of Sanjiao, and pathological products, such as phlegm dampness and blood stasis and other metabolic disorders. Therefore, the treatment should distinguish symptoms and essence. Starting from pathogenesis and pathological features of spleen and kidney deficiency and phlegm dampness and blood stasis, the treatment should flexibly apply the methods of nourishing spleen and kidney, reducing phlegm and dispelling humidity, and activating blood and using diuretic of hydragogue to alleviate water retention, which can greatly reduce side effects caused by the long-term use of Western medicine and the onset of gout, and then to help disease recovery.
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Objective To investigate the effects of the decoction of Huoxue Jiangtang Yin (HJY) combined with metformin on glucose and lipid metabolism and fat factors in type 2 diabetes patients with insulin resistance ( IR). Methods A total of 84 type 2 diabetes patients with IR were randomly assigned to experiment group and control group, 42 cases in each group. Both groups were given the basic treatment of controlling diet, exercise therapy, banning from smoking and alcohol, and psychological therapy. Additionally, the control group was given metformin orally, and the experiment group was given oral use of metformin and the decoction of HJY ( mainly composed of Radix Astragalie, Rhizoma Rehmanniae, Radix Salviae Miltiorrhizae, Radix Pseudostellariae, Fructus Schisandrae, Radix Ophiopogonis, Radix Dioscoreae, Rhizoma Polygonati, Cortex Moutan, Radix et Rhizoma Rhei, Flos Carthami, and Semen Persicae). The treatment for both groups covered 20 weeks for a course of treatment. Before and after the treatment, we observed the indexes of fasting plasma glucose (FPG), 2-h postprandial plasma glucose (2hPG), glycosylated hemoglobin (HbA1c), fasting insulin ( FINS) , homeostasis model assessment of insulin resistance ( HOMA-IR) , body mass index ( BMI) , blood lipid of total cholesterol ( TC) , triglyceride ( TG) and low density lipoprotein cholesterol ( LDL-C) , interleukin 6 ( IL -6) , tumor necrosis factor alpha ( TNF-α) , and secreted frizzled related protein 5 ( SFRP5). Results After treatment, FPG, 2hPG, HbA1c, BMI, FINS, TC, TG, LDL-C, HOMA-IR, TNF-α and IL -6 levels were all decreased (P<0.05 or P<0.01), and SFRP5 level was increased (P<0.05) in both groups compared with those before treatment. The improvement of the indexes in the experiment group was superior to that in the control group ( P<0.05 or P<0.01) Conclusion HJY combined with metformin has an effect on controlling body weight, regulating the glucose and lipid metabolism, and improving IR through regulating fat factors of IL-6, TNF-α and SFRP5 in type 2 diabetes patients with IR.
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Objective To observe and compare the incidence rate of hemolysis while venous blood specimen collecting with two types of venous indwelling needle. To summarize the attentions during blood samples collecting by venous indwelling needle. Methods From January 2014 to January 2015, a total of 400 patients in resuscitation room of emergency department were divided into control group and experimental group by random digits table method with 200 cases each. Type Y indwelling needle was used in control group, while straight type indwelling needle was used in experimental group. Both two types of indwelling needle were required to be connected with the disposable venous blood taking needle joint for blood sample collection. The incidence rate of hemolysis was observed. Results The incidence rate of hemolysis in experimental group was lower than that in control group: 8.0%(16/200) vs. 39.5%(79/200),and there was significant difference, χ2=34.317, P < 0.01. Conclusions The incidence rate of hemolysis during venous blood specimen collecting with straight type indwelling needle is significantly lower than that with Y type indwelling needle. Straight type indwelling needle is worthy of clinical use.
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Objective: The non-convulsive status epilepticus (NCSE) is easy misdiagnosed due to its complex etiology, clinical manifestations. It is more difficult to diagnose and treat for elderly patients with multiple basic diseases. The purpose of this study is to investigate the clinical characteristics of elderly patients with NCSE, therapy, diagnosis, and the value of long-range video electroencephalography (LV-EEG). Methods: The clinical and electroencephalography (EEG) data of the five elderly patients with NCSE in my hospital were analyzed retrospectively.Results: Five patients showed episodes of abnormal behavior during seizure (5/5). There were 3 patients with previous history of epilepsy after cerebral infarction (3/5), 1 patient diagnosed intracranial infection because of NCSE episodes to consultation (1/5), and 1 case with previous history of epilepsy (1/5). The V-EEG of the 5 patients showed persistent epileptic form discharges during seizure. Intravenous diazepam had good effect to the 5 patients during seizure. Seizure of the 5 cases was controlled acceptably with different antiepileptic drugs according to different epilepsy forms.Conclusions: The abnormal mind and behavior is still main performance in elderly patients with NCSE. The LV-EEG provides reliable foundation for diagnosis of the disease. The rational use of antiepileptic drugs is effective. Intravenous diazepam slow for elderly patients with NCSE is applied as well.
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BACKGROUND:In recent years, studies have shown that mesenchymal stem cells can secrete various growth factors, and has certain application prospect in the treatment of liver fibrosis. OBJECTIVE:To explore the different sources of mesenchymal stem cells in the treatment of liver fibrosis in rats. METHODS:Fifty Sprague-Dawley rats were given intraperitoneal injection of carbon tetrachloride to construct the model of liver fibrosis. Another 10 normal Sprague-Dawley rats were used as normal controls. Model rats were randomly divided into five groups, 10 rats in each group, including model control group, normal saline group, bone marrow mesenchymal stem cellgroup, umbilical cord mesenchymal stem cellgroup, and umbilical cord blood mesenchymal stem cellgroup. After 8 weeks of modeling, different sources of mesenchymal stem cells at a density of 2×106 were injected via tail vein into model rats. After 12 weeks, the rats were kil ed, and serum alanine aminotransferase, aspartate aminotransferase and albumin levels were detected as wel as the expression of type I col agen and glial fibril ary acidic protein in liver tissue was detected by fluorescence quantitative PCR method. RESULTS AND CONCLUSION:We have successful y established the SD rat model of hepatic fibrosis. Bone marrow mesenchymal stem cells aggravated hepatic fibrosis, umbilical cord blood and umbilical cord mesenchymal stem cells could reduce the level of hepatic fibrosis in rats. Umbilical cord mesenchymal stem cells had the most obvious effect that significantly reduced expressions of alanine aminotransferase, aspartate aminotransferase and type I col agen and glial fibril ary acidic protein. The results showed different sources of mesenchymal stem cells have different effects on rat fibrosis. Bone marrow mesenchymal stem cells aggravate hepatic fibrosis, and umbilical cord and umbilical cord blood stem cells can al eviate hepatic fibrosis in rats.
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BACKGROUND:How to obtain a sufficient number of cells is one of the key issues in the celltransplantation therapy, and studies have shown that stem cellproliferation can be promoted by reasonable stimulus. OBJECTIVE:To investigate reduced glutathione effects on biological characteristics of human umbilical cord blood mesenchymal stem cells. METHODS:The cells were divided into two groups:the control group consisted of the normal human umbilical cord blood mesenchymal stem cells, and in the experimental group, human umbilical cord blood mesenchymal stem cells were treated with 0.15 g/L reduced glutathione. RESULTS AND CONCLUSION:At days 5, 7, 9, cells treated with 0.15 g/L reduced glutathione showed higher absorbance values than those in the control group (P<0.05). Flow cytometry showed reduced glutathione had no effects on CD29, CD44, CD45, CD105 expression. Real-time PCR results showed reduced glutathione was capable of promoting extracellular signal-regulated kinase mRNA expression (P<0.05). Findings from this study showed that 0.15 g/L reduced glutathione can promote the proliferation of human umbilical cord blood mesenchymal stem cells.
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<p><b>UNLABELLED</b>To evaluate clinical efficacy of acupressure at Jiaji points for irritable bowel syndrome (IBS) and explore its pathogenesis and treatment law.</p><p><b>METHODS</b>Sixty outpatient cases of IBS from 2010 to 2012 were selected and randomly divided into an acupressure group and a medication group, 30 cases in each one. The acupressure at Jiaji points was applied in the acupressure group while pinaverium bromide tablet was orally prescribed in the medication group. The bowel symptom scale (BSS) was adapted to sore symptoms before and after the treatment, the improvement rate was adopted and results were analyzed.</p><p><b>RESULTS</b>The difference of BSS score in two groups after the treatment had statistical significance (both P<0.01). The efficacy in the acupressure group was superior to that in the medication group, which had significant difference (P<0.01). The improvement rate was 90.0% (27/ 30) in the acupressure group, which was better than 50.0% (15/30) in the medication group (P<0.01). The location of abdominal pain in IBS was lower abdomen while treatment location was from 11th thoracic vertebra to 1st lumbar vertebra.</p><p><b>CONCLUSION</b>The acupressure at Jiaji points could balance yin and yang in spine to quickly relive symptoms of IBS. The attack of IBS has some relationship with thoracic and lumbar vertebras.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Acupressure , Acupuncture Points , Irritable Bowel Syndrome , Therapeutics , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To assess the value of contrast-enhanced ultrasound imaging in monitoring the therapeutic effect of argon-helium cryosurgical treatment of malignant tumors.</p><p><b>METHODS</b>Before and after argon-helium cryosurgical treatment, 42 patients underwent contrast-enhanced ultrasound imaging, conventional ultrasound imaging and enhanced CT or magnetic resonance imaging (MRI) for examining the number of tumor foci and the size of necrotic areas.</p><p><b>RESULTS</b>A total of 80 tumor lesions were detected by contrast-enhanced ultrasound imaging. Compared with conventional ultrasound imaging, contrast-enhanced ultrasound imaging detected a significantly greater number of tumors and the intratumoral necrotic areas (96 vs 19) as well as a significantly increased mean size of necrotic areas (5.7∓3.6 cm vs 2.8∓1.7 cm), showing no significant differences from the results by enhanced CT and MRI (94 and 5.5∓3.3 cm, P=0.872 and 0.978, respectively). The short-term therapeutic effect of argon-helium cryosurgery evaluated by contrast-enhanced ultrasound imaging were also similar to that assessed by enhanced CT or MRI (P=0.906).</p><p><b>CONCLUSION</b>Contrast-enhanced ultrasound imaging has important values in monitoring malignant tumors during argon-helium cryosurgical treatment and in evaluating the short-term therapeutic effect of the treatment.</p>
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Adult , Aged , Female , Humans , Male , Middle Aged , Argon , Cryosurgery , Methods , Helium , Neoplasms , Diagnostic Imaging , General Surgery , Treatment Outcome , UltrasonographyABSTRACT
<p><b>OBJECTIVE</b>To explore the cause and mechanism of irritable bowel syndrome, and to study the relationship between irritable bowel syndrome and unstable thoracolumbar vertebrae.</p><p><b>METHODS</b>From 2004 to 2006, 82 patients with irritable bowel syndrome were treated with manipulation thoracolumbar vertebrae without any drug using. Among the patients, 24 patients were male and 58 patients were female, ranging in age from 15 to more than 61 years.</p><p><b>RESULTS</b>After the treatment, 75 patients were cured and 7 patients improved.</p><p><b>CONCLUSION</b>Unstable thoracolumbar vertebrae is the cause of irritable bowel syndrome. It is a simple and effective way by manipulation on thoracolumbar vertebrae to release compression and stimulation on peripheral nerve and vascular for treating this disease.</p>
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Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Irritable Bowel Syndrome , Lumbar Vertebrae , Manipulation, Spinal , Spinal Diseases , Thoracic VertebraeABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between nucleotide excision repair gene ERCC1 and resistance to cisplatin in ovarian cancer.</p><p><b>METHODS</b>The expression of gene ERCC1 in 58 ovarian cancer tissues and 4 cell lines were examined and its relationship with resistance to cisplatin were analyzed, the changes of sensitivity to cisplatin were observed after interference of ERCC1 gene with small interfering RNA (siRNA) in ovarian cancer cell lines.</p><p><b>RESULTS</b>In 58 ovarian cancer tissues, the positive rate of ERCC1 protein in chemoresistant cases (57.89%) was higher than that in chemo-sensitive cases (28.21%, P = 0.029). The mRNA levels of ERCC1 gene in ovarian cancer cell lines ES-2, SKOV3, COC1, COC1/DDP were related to cisplatin IC50 values (r = 0.932, P <0.05). The sensitivity of cell lines ES-2, SKOV3, COC1/DDP cells to cisplatin was increased by 53.88, 5.07, and 3.75 times, respectively, after RNA interfering ERCC1 gene.</p><p><b>CONCLUSION</b>ERCC1 gene is associated with the resistance to cisplatin and the sensitivity to cisplatin can be enhanced by RNA interfering ERCC1 in ovarian cancer.</p>